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Aisea e mana'omia ai e ipu fa'aleaganu'u sela ni togafitiga i luga ole laiga?

2023-08-16

Ina ia faʻamalieina manaʻoga o faʻataʻitaʻiga aganuʻu aganuʻu ma ia ausia taunuʻuga manaʻomia, e masani ona matou faʻaogaina togafitiga TC, togafitiga faʻaleleia TC ma togafitiga faʻapipiʻi ultra-maualalo mo sela taofia.


1. TC togafitiga, talafeagai mo le aganuu o sela pipii

Faʻatasi ai ma togafitiga faʻapitoa kasa gas plasma, e mafai ona faʻaauau pea ma tutusa le faʻapipiʻiina o le vaega i luga ole laiga i vaega lelei ma le le lelei mo se taimi umi, lea e faʻamautinoa ai se faʻapipiʻi sili atu ma mautu. O le faʻaofiina o le faʻaluaina o totogi e faia ai le TC mo le endothelial, hepatocyte ma le neuron cell culture e sili atu le faʻapipiʻiina ma faʻasalalau nai lo luga ole TC tutusa, ma e mafai ona ausia le faʻatinoga sili ona lelei o le faʻaogaina o le cell e fetaui ma le aganuu o se maualuga maualuga o sela pipii. O luga e mafai ona ausia le sili ona lelei le faʻaogaina o le faʻaogaina o le cell ma faʻafeiloaʻi le maualuga maualuga o le aganuʻu cell adherent.


2. Togafitiga faʻaleleia TC, talafeagai mo Kulu Cell ma Manaoga Faʻapipiʻi Maualuga.

Togafitiga aganuʻu aganuʻu faʻapitoa, faʻatusatusa i oloa faʻapitoa TC-togafitiga, TC-faʻaleleia luga o loʻo i ai le malosi e faʻaleleia ai le faʻapipiʻiina ma le faʻalauteleina, faʻavavevave le faʻalauteleina o le faitau aofaʻi o tagata, e mafai ona faʻaaogaina mo le faʻalauteleina o sela manaʻomia, e pei o sela muamua poʻo maaleale, faʻapea foʻi ma sela e faʻatupuina i lalo o tulaga faʻasaina o le tuputupu aʻe (serum-free poʻo le faʻaitiitia o le serum), faʻateleina le faʻalauteleina o le faitau aofaʻi o tagata, faʻalauiloaina o le pipii ma le faʻalauteleina, e mafai ona teuina i le vevela o le potu mo le tele o faʻaoga.


3. Ultra-maualalo adsorption fa'asologa mo le fa'amalologa tu ma aga

Fa'apitoa amphoteric molecular polymer o lo'o ufiufi i luga o le va'a fa'aleaganu'u. Talu ai ona o lenei tuufaatasiga e faapitoa lava le hydrophilic, e mafai e le amphoteric molecules ona faʻapipiʻi mole vai e fausia ai se puipui o le vai, ina ia le mafai ona pipii atu sela, molelaʻau polotini, siama ma isi mea i le vaʻa aganuu, e mafua ai le ultra-low cell adherence, lea. e mafai ona faʻatupuina i le faʻamalolo mo le sili atu i le 15 aso.

E mafai ona faʻaaogaina mo le aganuʻu o sela fetal, hemocytes ma isi sela e manaʻomia le tuputupu aʻe i totonu o le aganuu faʻamalolo, faʻapea foʻi ma le faʻalauiloaina o le aganuu o sela 3D spheroid ma organoids, ma e iai mea faʻapipiʻi faʻamalosi i sela pipii malosi.


O lo'o ofoina atu e Cotaus le atoaga o oloa gaosi oloa, mo nisi fa'amatalaga o oloa, fa'amolemole fa'afeso'ota'i matou.


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